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KMID : 0380620070390020209
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Korean Journal of Food Science and Technology
2007 Volume.39 No. 2 p.209 ~ p.216
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Glycoprotein Isolated from Morus indica Linne Has an Antioxidative Activity and Inhibits Signal Factors Induced by Bisphenol A in Raw 264.7 Cells
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Shim Jae-Uoong
Lee Sei-Jung
Oh Phil-Sun
Lim Kye-Taek
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Abstract
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The present study investigated anti-oxidative and anti-inflammatory activity of glycoprotein isolated from Morus Indica Linne (MIL glycoprotein). We found that MIL glycoprotein has a molecular weight of 32 kD and consists of carbohydrate (40.03%) and protein (59.97%), and that it has a strong scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical , and superoxide anion radicals. In addition, MIL glycoprotein had a stable character and an optimal DPPH radical scavenging activity in the alkaline and neutral pH solution, and up to at 105. However, the results indicated that it has a minimal scavenging activity in the metal ionic solution (, , and ) in the presence of EDTA. In addition, we further investigated whether MIL glycoprotein scavenges oxygen radicals and blocks inflammation-related signals in the bisphenol A (BPA)-stimulated Raw 264.7 cells. The results in this study showed that it has a character to scavenge the productions of reactive oxygen species (ROS) and nitric oxide (NO) dose-dependently. Also it blocked the activities of inflammation-related signals such as nuclear factor-kappa B () and inducible nitric oxide synthase (iNOS). For example, it had an inhibitory effect on the activation of (p50) and iNOS proteins at 200 MIL glycoprotein. Here, we speculate that MIL glycoprotein is one of natural antioxidants and of modulators of the BPA-induced inflammation.
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KEYWORD
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Morus Indica Linne, Glycoprotein, Antioxidant, Bisphenol A, RAW 264.7 cells
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